|Statement||edited by Pauline M. Doran.|
|Contributions||Doran, Pauline M.|
|LC Classifications||QK644 .H35 1997|
|The Physical Object|
|Pagination||256 p. :|
|Number of Pages||256|
A contributory volume presenting conceptual information on hairy root culture systems based on the latest research. Hairy root biotechnology is recognized as most sought after and a dynamic research area. The book explores hairy roots with the help of relevant case studies. Hairy Roots: /ch Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced . Hairy root culture is an interdisciplinary science, with important and expansive applications. This volume is the first to be dedicated solely to the many facets of hairy root culture. The number of papers dedicated to hairy roots is rising exponentially, and with the increasing amount of research already underway this forms a timely publication. This book provides detailed conceptual and practical information on HRC-based research, along with relevant case studies. The content is divided into three broad sections, namely (i) Hairy Roots and Secondary Metabolism, (ii) Progressive Applications, and (iii) Novel Approaches and Future Prospects.
1 day ago Hairy root culture is an interdisciplinary science, with important and expansive applications. This volume is the first to be dedicated solely to the many facets of hairy root culture. The number of papers dedicated to hairy roots is rising exponentially, and with the increasing amount of research already underway this forms a timely publication. Hairy root culture, also called transformed root culture, is a type of plant tissue culture that is used to study plant metabolic processes or to produce valuable secondary metabolites or recombinant proteins, often with plant genetic engineering.. A naturally occurring soil bacterium Agrobacterium rhizogenes that contains root-inducing plasmids (also called Ri plasmids) can infect plant roots. hairy roots at the infection site. Hairy roots can be excised, cleared of excess bacteria using antibiotics, and grown indefinitely in vitro by subculture of root tips in liquid medium. Practical techniques for initiation, culture, genetic manipulation, and molecular analysis of hairy roots are summarized in Ham- ill and Lidgett (1). Identification. R. rhizogenes is a capsule forming gram-negative bacterium with a polar flagellum. R. rhizogenes was first identified based on the ability to induce hairy root growth in plants. In the ’s, it was discovered that gene transmission into the plant via the Ri-plasmid is responsible for hairy root growth and that the Ri-plasmids are highly variable amongst strains.
Scaling up of hairy roots in novel bioreactors can provide the best conditions for optimum growth and secondary metabolite production than in native root culture. Hairy root morphology is quite plastic so the bioreactor design for root culture is a balancing act between plant . Kawauchi M, Arima T, Shirota O, Sekita S, Nakane T, Takase Y, Kuroyanagi M () Production of sesquiterpene-type phytoalexins by hairy roots of Hyoscyamus albus co-treated with copper sulfate and methyl jasmonate. Chem Pharm Bull – PubMed CrossRef Google Scholar. Emphasis has focused on designing appropriate bioreactors suitable to culture the delicate and sensitive plant hairy roots. Recent reactors used for mass production of hairy roots can roughly be divided as liquid-phase, gas-phase, or hybrid reactors. The present review highlights the nature, applications, perspectives and scale up of hairy root. Plant hairy roots offer a novel and sustainable tissue-based system that preserves multiple specialized cell types believed to be important in maintaining a better consistency in synthesis of.